4XA9
Crystal structure of the complex between the N-terminal domain of RavJ and LegL1 from Legionella pneumophila str. Philadelphia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 219.608, 120.885, 149.288 |
Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
Resolution | 29.943 - 2.000 |
R-factor | 0.1595 |
Rwork | 0.158 |
R-free | 0.19460 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.854 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.043 | 0.208 |
Number of reflections | 258259 | |
<I/σ(I)> | 21.14 | 4.82 |
Completeness [%] | 97.8 | 94.4 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M NaCl, 100 mM Tris pH 8.5 and 26% (w/v) PEG3350 |