4XA6
Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-09 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.546, 46.889, 169.055 |
Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
Resolution | 43.887 - 3.420 |
R-factor | 0.2419 |
Rwork | 0.239 |
R-free | 0.29670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1no4 1yib |
RMSD bond length | 0.006 |
RMSD bond angle | 0.925 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 15120 | |
<I/σ(I)> | 14.8 | 4.8 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES) |