4XA3
Crystal structure of the coiled-coil surrounding Skip 2 of MYH7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.462, 146.115, 153.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.726 - 2.548 |
| R-factor | 0.269 |
| Rwork | 0.267 |
| R-free | 0.31140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no4 1yib |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1819)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 11019 | |
| <I/σ(I)> | 16.3 | 2.2 |
| Completeness [%] | 87.0 | 53 |
| Redundancy | 6.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol |
