4XA2
Structure of the Major Type IV pilin of Acinetobacter baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.018, 128.300, 92.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.020 - 1.980 |
Rwork | 0.181 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XDS |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.250 | 46.250 | 2.020 |
High resolution limit [Å] | 1.980 | 9.050 | 1.980 |
Rmerge | 0.232 | 0.212 | 1.552 |
Rpim | 0.095 | 0.090 | 1.101 |
Total number of observations | 362459 | 4820 | 2442 |
Number of reflections | 53332 | ||
<I/σ(I)> | 12.3 | 19.5 | 2 |
Completeness [%] | 79.7 | 99.1 | 19.6 |
Redundancy | 6.8 | 6.6 | 2.7 |
CC(1/2) | 0.983 | 0.872 | 0.110 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Bis-Tris, 22.5% PEG 3350, 0.3M 1,6 Hexandediol |