4X9X
Biochemical Roles for Conserved Residues in the Bacterial Fatty Acid Binding Protein Family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.343, 41.872, 38.573 |
Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
Resolution | 26.094 - 1.199 |
R-factor | 0.1189 |
Rwork | 0.118 |
R-free | 0.14400 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.100 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.357 | |
Number of reflections | 78877 | |
<I/σ(I)> | 27.4 | 2.4 |
Completeness [%] | 94.5 | 77 |
Redundancy | 4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 0.1 M Citrate pH 5.5, 20% PEG 3000 |