4X9P

Crystal structure of bovine Annexin A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-14
Detector	SBC-3
Wavelength(s)	0.97901
Spacegroup name	P 21 21 21
Unit cell lengths	48.315, 63.274, 117.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.010
R-factor	0.1592
Rwork	0.157
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xjl
RMSD bond length	0.017
RMSD bond angle	1.753
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.010	4.330	2.010
Rmerge	0.048	0.025	0.276
Total number of observations	168541
Number of reflections	24564
<I/σ(I)>	12.1		5.9
Completeness [%]	99.7	98.9	99.1
Redundancy	6.9	6.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	Reservoir buffer: 30% PEG 4000, 0.1 M Na acetate, 0.2 M ammonium acetate. The drop consisted of 0.5 mkl of 2.3 mg/ml protein solution + 0.5 mkl of reservoir buffer supplemented with 20 mkM Ca2+ and 1 mM Mg2+.