4X9M
Oxidized L-alpha-Glycerophosphate Oxidase from Mycoplasma pneumoniae with FAD bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9765 |
Spacegroup name | P 2 3 |
Unit cell lengths | 111.590, 111.590, 111.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.900 - 2.400 |
R-factor | 0.15044 |
Rwork | 0.148 |
R-free | 0.20376 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dme |
RMSD bond length | 0.015 |
RMSD bond angle | 1.802 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 18401 | |
<I/σ(I)> | 22.7 | 4.4 |
Completeness [%] | 100.0 | 99 |
Redundancy | 23.4 | 23.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 2.68 M NaCl, 2% v/v isopropanol, 0.1 M HEPES pH 7.5 |