4X9L

N-terminal domain of Heat shock protein 90 from Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.953725
Spacegroup name	I 4 2 2
Unit cell lengths	116.870, 116.870, 105.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.416 - 3.100
R-factor	0.2475
Rwork	0.245
R-free	0.29170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jki
RMSD bond length	0.009
RMSD bond angle	1.453
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.440	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.133	0.836
Number of reflections	6890
<I/σ(I)>	12.8	3
Completeness [%]	100.0	100
Redundancy	11	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1M Tris, PEG 400, Ammonium sulphate