4X9I
Crystal structure of Dscam1 isoform 9.44, N-terminal four Ig domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.746, 56.673, 128.688 |
Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
Resolution | 45.490 - 2.904 |
R-factor | 0.2209 |
Rwork | 0.218 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v5m |
RMSD bond length | 0.003 |
RMSD bond angle | 0.842 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 20863 |
<I/σ(I)> | 8.9 |
Completeness [%] | 96.6 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.1M Sodium acetate, pH 4.6, 25% (w/v) PEG 1500 |