4X9H
Crystal structure of Dscam1 isoform 8.4, N-terminal four Ig domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.222, 60.950, 92.351 |
| Unit cell angles | 90.00, 93.56, 90.00 |
Refinement procedure
| Resolution | 47.519 - 2.950 |
| R-factor | 0.2744 |
| Rwork | 0.272 |
| R-free | 0.32350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 22498 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 97.2 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M MES pH6.5, 20% (w/v) PEG 3350 |
