4X9G
Crystal structure of Dscam1 isoform 6.44, N-terminal four Ig domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.639, 63.416, 90.073 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 48.587 - 3.403 |
| R-factor | 0.2518 |
| Rwork | 0.248 |
| R-free | 0.31720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.812 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 14910 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 98.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M HEPES pH 7.5, 15% (w/v)PEG 3350 |
