4X9F

Crystal structure of Dscam1 isoform 6.9, N-terminal four Ig domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.686, 90.500, 169.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.305 - 2.350
R-factor	0.2134
Rwork	0.211
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v5m
RMSD bond length	0.004
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	42006
<I/σ(I)>	27.7
Completeness [%]	99.7
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M HEPES pH7.5, 0.2M Ammonium Sulfate, 25% (w/v) PEG 3350