4X9E
DEOXYGUANOSINETRIPHOSPHATE TRIPHOSPHOHYDROLASE from Escherichia coli with two DNA effector molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 136.800, 159.800, 190.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.632 - 3.100 |
R-factor | 0.1697 |
Rwork | 0.167 |
R-free | 0.22290 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.173 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.725 | |
Number of reflections | 76093 | |
<I/σ(I)> | 15.1 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 283 | Protein solution (10 mg/mL dGTPase, 20 mM Tris-HCl, pH 7.0) mixed 1:1 with well solution (0.1 M sodium acetate, 8% w/v polyethylene glycol 4000, pH 4.6). Cryo conditions are well solution at 25% ethylene glycol. |