4X9B
Crystal structure of Dscam1 isoform 4.44, N-terminal four Ig domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.902, 59.815, 89.560 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 48.850 - 2.200 |
| R-factor | 0.2087 |
| Rwork | 0.207 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 45397 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.7 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M HEPES pH 7.5, 0.1M Calcium Chloride, 30%(w/v) PEG 400 |
