4X8W
dsRBD3 of Loquacious
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.018, 112.970, 114.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.453 - 2.647 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.647 | 2.650 |
| Rmerge | 0.035 | |
| Number of reflections | 25522 | |
| <I/σ(I)> | 33.8 | 6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% PEG1000 12% PEG8000 50 mM Hepes-NaOH 250 mM NaCl 1mM DTT |
