4X8F

Vibrio cholerae O395 Ribokinase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-18
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.973
Spacegroup name	P 1
Unit cell lengths	129.220, 130.849, 145.690
Unit cell angles	110.52, 90.00, 119.59

Refinement procedure

Resolution	43.570 - 3.400
R-factor	0.2422
Rwork	0.239
R-free	0.30790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rk2
RMSD bond length	0.012
RMSD bond angle	1.840
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	3.580
High resolution limit [Å]	3.400	3.400
Rmerge		0.460
Number of reflections	97045
<I/σ(I)>	4.7	2
Completeness [%]	92.9	85.6
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	5% PEG 6000, 0.1M MES