4X8A
NavMS pore and C-terminal domain grown from protein purified in LiCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 199 |
| Detector technology | PIXEL |
| Collection date | 2013-04-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.81568 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.090, 332.350, 80.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.560 - 3.020 |
| R-factor | 0.2188 |
| Rwork | 0.217 |
| R-free | 0.24420 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.560 | 3.200 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.204 | 0.772 |
| Number of reflections | 21578 | |
| <I/σ(I)> | 10.4 | 3.4 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M NA3CITRATE, 0.1 M TRIS, PH 8.0, 34% PEG400 |
