4X89
NavMs voltage-gated sodium channal pore and C-terminal domain soaked with Silver nitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.000, 329.240, 80.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.250 - 2.620 |
| R-factor | 0.2289 |
| Rwork | 0.228 |
| R-free | 0.24470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zjz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.310 | 2.740 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.191 | 0.757 |
| Number of reflections | 32142 | |
| <I/σ(I)> | 7.5 | 2.7 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M NA3CITRATE, 0.1 M TRIS, PH 8.0, 34% PEG400 |
