4X88
E178D Selectivity filter mutant of NavMS voltage-gated pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.230, 336.020, 80.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 3.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zjz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.251 | 0.545 |
| Number of reflections | 14175 | |
| <I/σ(I)> | 7.7 | 3.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M NA3CITRATE, 0.1 M TRIS, PH 8.0, 34% PEG400 |
