4X86

Crystal structure of BAG6-Ubl4a complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1
Spacegroup name	P 62
Unit cell lengths	72.987, 72.987, 48.481
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.469 - 1.850
R-factor	0.2075
Rwork	0.204
R-free	0.23710
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.150
Data reduction software	Aimless (0.1.27)
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.480	48.480	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.045	0.027	3.491
Rpim	0.011	0.006	0.802
Total number of observations	249572	2145	15645
Number of reflections	12717
<I/σ(I)>	31.6	109.9	1.1
Completeness [%]	100.0	98.9	100
Redundancy	19.6	17.9	19.8
CC(1/2)	1.000	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.6	293	CAPS-NaOH, AmSO4, LiSO4, 2,2,2-trifluoroethanol, CHAPS