4X7P
Crystal structure of apo S. aureus TarM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 63 |
| Unit cell lengths | 208.599, 208.599, 120.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.330 - 3.400 |
| R-factor | 0.1733 |
| Rwork | 0.173 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.330 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 59887 |
| <I/σ(I)> | 8.8 |
| Completeness [%] | 100.0 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 200 mM Na/K tartrate, 100 mM tri-sodium citrate pH 6.5 and 2.1 M ammonium sulfate |
