4X7G
CobK precorrin-6A reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.090, 60.280, 76.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.290 - 1.220 |
R-factor | 0.17809 |
Rwork | 0.177 |
R-free | 0.19702 |
Structure solution method | SAD |
RMSD bond length | 0.029 |
RMSD bond angle | 2.831 |
Data reduction software | XSCALE |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 1.220 |
High resolution limit [Å] | 1.220 | 1.000 |
Rmerge | 0.138 | 0.596 |
Number of reflections | 73880 | |
<I/σ(I)> | 16.254 | 6.6 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 7.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 18% w/v PEG 3350, 0.2 M NH4 Cl |