4X6A

Crystal structure of yeast RNA polymerase II encountering oxidative Cyclopurine DNA lesions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	156.550, 220.334, 191.563
Unit cell angles	90.00, 97.00, 90.00

Refinement procedure

Resolution	50.000 - 3.960
R-factor	0.2513
Rwork	0.249
R-free	0.29640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.484
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.020
High resolution limit [Å]	3.950	10.690	3.950
Rmerge	0.255	0.084	0.937
Rmeas	0.271	0.092
Rpim	0.120	0.037	0.455
Total number of observations	278450
Number of reflections	54937
<I/σ(I)>	3.4
Completeness [%]	98.4	100	97.7
Redundancy	5.1	5.7	4.7
CC(1/2)		0.995	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	390 mM (NH4)2HPO4/NaH2PO4, PH 5.9-6.3, 50 mM dioxane, 10 mM DTT, and 10.7% - 11.6% PEG6000