4X6A
Crystal structure of yeast RNA polymerase II encountering oxidative Cyclopurine DNA lesions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.550, 220.334, 191.563 |
Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.960 |
R-factor | 0.2513 |
Rwork | 0.249 |
R-free | 0.29640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.484 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.020 |
High resolution limit [Å] | 3.950 | 10.690 | 3.950 |
Rmerge | 0.255 | 0.084 | 0.937 |
Rmeas | 0.271 | 0.092 | |
Rpim | 0.120 | 0.037 | 0.455 |
Total number of observations | 278450 | ||
Number of reflections | 54937 | ||
<I/σ(I)> | 3.4 | ||
Completeness [%] | 98.4 | 100 | 97.7 |
Redundancy | 5.1 | 5.7 | 4.7 |
CC(1/2) | 0.995 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 390 mM (NH4)2HPO4/NaH2PO4, PH 5.9-6.3, 50 mM dioxane, 10 mM DTT, and 10.7% - 11.6% PEG6000 |