4X66

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	401.322, 401.322, 175.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.778 - 3.446
R-factor	0.1955
Rwork	0.194
R-free	0.22670
RMSD bond length	0.005
RMSD bond angle	0.760
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1938))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.000	55.000	3.510
High resolution limit [Å]	3.446	9.350	3.455
Rmerge	0.188	0.067
Rmeas	0.210	0.074
Rpim	0.094	0.031	0.650
Total number of observations	930760
Number of reflections	181074
<I/σ(I)>	6.2
Completeness [%]	96.6	90.7	98.7
Redundancy	5.1	5.2	4.9
CC(1/2)		0.992	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD