4X65

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	401.440, 401.440, 175.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.959 - 3.345
R-factor	0.1854
Rwork	0.184
R-free	0.21040
RMSD bond length	0.005
RMSD bond angle	0.779
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1938))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.410
High resolution limit [Å]	3.345	9.040	3.345
Rmerge	0.135	0.040
Rmeas	0.141	0.042
Rpim	0.043	0.012	0.362
Total number of observations	2438566
Number of reflections	204935
<I/σ(I)>	6.5
Completeness [%]	100.0	99.7	100
Redundancy	11.9	12.2	9.2
CC(1/2)		0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD