4X65
Crystal Structure of 30S ribosomal subunit from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 401.440, 401.440, 175.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.959 - 3.345 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.21040 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.779 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1938)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 3.410 |
High resolution limit [Å] | 3.345 | 9.040 | 3.345 |
Rmerge | 0.135 | 0.040 | |
Rmeas | 0.141 | 0.042 | |
Rpim | 0.043 | 0.012 | 0.362 |
Total number of observations | 2438566 | ||
Number of reflections | 204935 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 11.9 | 12.2 | 9.2 |
CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD |