4X64
Crystal Structure of 30S ribosomal subunit from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 400.496, 400.496, 175.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.878 - 3.350 |
R-factor | 0.1668 |
Rwork | 0.165 |
R-free | 0.20490 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.821 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1938)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 3.410 |
High resolution limit [Å] | 3.350 | 9.040 | 3.350 |
Rmerge | 0.119 | 0.077 | 0.819 |
Rmeas | 0.130 | 0.084 | 0.993 |
Rpim | 0.052 | 0.032 | 0.549 |
Total number of observations | 1130734 | ||
Number of reflections | 195543 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 96.4 | 96.4 | 91.4 |
Redundancy | 5.8 | 6.5 | 2.7 |
CC(1/2) | 0.991 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MPD |