4X64

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	400.496, 400.496, 175.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.878 - 3.350
R-factor	0.1668
Rwork	0.165
R-free	0.20490
RMSD bond length	0.004
RMSD bond angle	0.821
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1938))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.410
High resolution limit [Å]	3.350	9.040	3.350
Rmerge	0.119	0.077	0.819
Rmeas	0.130	0.084	0.993
Rpim	0.052	0.032	0.549
Total number of observations	1130734
Number of reflections	195543
<I/σ(I)>	8
Completeness [%]	96.4	96.4	91.4
Redundancy	5.8	6.5	2.7
CC(1/2)		0.991	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD