4X5O

Human histidine tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	93
Detector technology	CCD
Collection date	2012-06-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 41 21 2
Unit cell lengths	97.669, 97.669, 254.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.021 - 2.800
R-factor	0.2518
Rwork	0.248
R-free	0.31360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kmm
RMSD bond length	0.004
RMSD bond angle	0.905
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	31689
<I/σ(I)>	16.4
Completeness [%]	97.0
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.29 mM potassium sodium tartrate tetrahydrate, 18 % polyethylene glycol 3350