4X5J
ecDHFR complexed with folate and NADP+ at 750 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-01-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.71 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.880, 58.594, 38.201 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 36.540 - 1.850 |
| R-factor | 0.20598 |
| Rwork | 0.203 |
| R-free | 0.26448 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.044 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 13475 |
| <I/σ(I)> | 16.7 |
| Completeness [%] | 98.3 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 6000, imidazole, calcium chloride |
