4X5G
ecDHFR complexed with folate and NADP+ at 270 MPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2013-04-25 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.75 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.721, 59.366, 71.700 |
Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
Resolution | 37.830 - 1.900 |
R-factor | 0.15734 |
Rwork | 0.155 |
R-free | 0.20853 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.085 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 25274 |
<I/σ(I)> | 29.3 |
Completeness [%] | 91.8 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 6000, imidazole, calcium chloride |