4X4X
Retrofitting antibodies with stabilizing mutations. Herceptin scFv mutant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 91.190, 91.190, 114.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.650 - 2.250 |
R-factor | 0.2283 |
Rwork | 0.226 |
R-free | 0.26906 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.451 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.650 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.104 | 0.860 |
Number of reflections | 26621 | |
<I/σ(I)> | 8.5 | 1.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 3.5 M sodium formate, 100 mM sodium acetate (pH 4.5) |