4X4M
Structure of FcgammaRI in complex with Fc reveals the importance of glycan recognition for high affinity IgG binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 59.921, 67.991, 125.028 |
Unit cell angles | 89.86, 112.25, 89.96 |
Refinement procedure
Resolution | 40.665 - 3.485 |
R-factor | 0.2493 |
Rwork | 0.245 |
R-free | 0.29570 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.717 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.485 | 3.485 |
Rmerge | 0.135 | 0.650 |
Number of reflections | 20950 | |
<I/σ(I)> | 22.1 | 1 |
Completeness [%] | 91.5 | 68.8 |
Redundancy | 5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG8000, 10mM Hepes(pH7.5), 50mM Lithium sulfate |