4X3S

Crystal structure of chromobox homology 7 (CBX7) with SETDB1-1170me3 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-08
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.027, 46.040, 80.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.260 - 1.600
R-factor	0.16506
Rwork	0.162
R-free	0.22154
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i91
RMSD bond length	0.018
RMSD bond angle	1.737
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.400
High resolution limit [Å]	1.600
Number of reflections	23204
<I/σ(I)>	42.2
Completeness [%]	99.4
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	290	10% Jeffamine M-600, 0.1 M Na Citrate pH 5.6, 0.01 M FeCl3