4X3Q

Crystal structure of S-adenosylmethionine-dependent methyltransferase SibL in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	91.787, 112.113, 143.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.150 - 2.586
R-factor	0.1863
Rwork	0.184
R-free	0.24820
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.385
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.586
Number of reflections	46368
<I/σ(I)>	36.6
Completeness [%]	98.9
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	277	0.1 M HEPES (pH 7.7), 2.0 M (NH4)2SO4, and 2% PEG400