4X3N

Crystal structure of 34 kDa F-actin bundling protein from Dictyostelium discoideum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	78.161, 85.244, 135.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.190 - 1.890
R-factor	0.20643
Rwork	0.204
R-free	0.25237
RMSD bond length	0.017
RMSD bond angle	1.740
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.190	1.930
High resolution limit [Å]	1.890	1.890
Number of reflections	72226
<I/σ(I)>	34.5
Completeness [%]	98.9	88.2
Redundancy	9.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	ammonium acetate, sodium citrate, PEG 4000