4X3I
The crystal structure of Arc N-lobe complexed with CAMK2A fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-11-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9801 |
Spacegroup name | P 65 |
Unit cell lengths | 51.527, 51.527, 69.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.760 - 1.800 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.763 | |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 9807 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2M AMMONIUM SULFATE AND 0.1 M TRIS, PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |