4X39

Gallus interleukin-1 beta mutant - T117A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	35.613, 38.509, 102.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.160 - 1.610
R-factor	0.2023
Rwork	0.200
R-free	0.23655
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wry
RMSD bond length	0.015
RMSD bond angle	1.762
Data scaling software	DENZO (HKL-2000)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	26.160
High resolution limit [Å]	1.600
Number of reflections	19075
<I/σ(I)>	39.14
Completeness [%]	99.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.15 M (NH4)2SO4, 0.1 M Tris, 0.2 M LiSO4