4X38

Gallus interleukin-1 beta mutant - E118A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	35.670, 35.758, 102.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.660 - 2.120
R-factor	0.19251
Rwork	0.190
R-free	0.24716
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wry
RMSD bond length	0.010
RMSD bond angle	1.456
Data scaling software	DENZO
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.660
High resolution limit [Å]	2.120
Number of reflections	7853
<I/σ(I)>	13.6
Completeness [%]	98.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.15 M (NH4)2SO4, 0.1 M Tris, 0.2 M LiSO4