4X37

Gallus interleukin-1 mutant - E118K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	35.576, 102.501, 37.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 1.630
R-factor	0.19915
Rwork	0.198
R-free	0.22893
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wry
RMSD bond length	0.025
RMSD bond angle	2.457
Data scaling software	DENZO (HKL-2000)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.200
High resolution limit [Å]	1.630
Number of reflections	17881
<I/σ(I)>	66.87
Completeness [%]	98.7
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.15 M (NH4)2SO4, 0.1 M Tris, 0.2 M LiSO4