4X33

Structure of the Elongator cofactor complex Kti11/Kti13 at 1.45A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-02
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	C 2 2 21
Unit cell lengths	49.790, 89.009, 163.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.230 - 1.450
R-factor	0.1511
Rwork	0.150
R-free	0.17620
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Refinement software	BUSTER-TNT (2.10.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.500	44.500	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.105	0.046	2.145
Rpim	0.044	0.021	0.875
Total number of observations	431552	2589	20863
Number of reflections	64706
<I/σ(I)>	9.5	35.6	0.8
Completeness [%]	99.8	98.9	97.3
Redundancy	6.7	5.6	6.8
CC(1/2)	0.998	0.998	0.275

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	22.5% PEG 3350, 0.2 MgCl2 and 0.1M Tris pH 8