4X32

Bacteriorhodopsin ground state structure collected in cryo conditions from crystals obtained in LCP with PEG as a precipitant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-15
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	60.525, 60.525, 101.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.420 - 1.900
R-factor	0.1737
Rwork	0.172
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ntu
RMSD bond length	0.009
RMSD bond angle	1.223
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.570	46.570	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.095	0.021	1.867
Rpim	0.026	0.006	0.496
Total number of observations	234541	2117	15314
Number of reflections	16643
<I/σ(I)>	17.9	68.6	1.8
Completeness [%]	100.0	99.4	100
Redundancy	14.1	12.9	14.3
CC(1/2)	1.000	1.000	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	294	27% PEG2000, 100 mM phosphate buffer pH 5.6