4X2S
Crystal structure of 276S/M395R-GltPh in inward-facing conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.651, 121.651, 578.699 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.210 |
| R-factor | 0.27964 |
| Rwork | 0.278 |
| R-free | 0.31363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nwx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 4.200 |
| Number of reflections | 15575 |
| <I/σ(I)> | 23 |
| Completeness [%] | 82.0 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 50 mM sodium acetate, pH 5.6-6, 18-20 % PEG 400, 100-150 mM magnesium acetate |
