4X2O

Sac3N peptide bound to Mex67:Mtr2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	56.724, 73.007, 93.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.453 - 1.850
R-factor	0.1637
Rwork	0.162
R-free	0.20120
RMSD bond length	0.010
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.453
High resolution limit [Å]	1.850
Number of reflections	33498
<I/σ(I)>	15.5
Completeness [%]	99.3
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20 % PEG 6000, 1M LiCl, 0.1M MES pH 6.0. See manuscript for details.