4X2H

Sac3N peptide bound to Mex67:Mtr2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	53.019, 74.195, 54.816
Unit cell angles	90.00, 112.87, 90.00

Refinement procedure

Resolution	44.900 - 1.800
R-factor	0.1654
Rwork	0.164
R-free	0.18700
RMSD bond length	0.004
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	1.840
High resolution limit [Å]	1.800	1.800
Rmerge		1.010
Number of reflections	36313
<I/σ(I)>	9.3	1.8
Completeness [%]	99.9	99.6
Redundancy	4.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	30% PEG 400, 0.1M NaCl, 0.1M MgCl2, 0.1 M Na Citrate, pH 5.5. See publication for complete details.