4X29
Structural basis for the enhancement of virulence by entomopoxvirus fusolin and its in vivo crystallization into viral spindles (complex with Zinc)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.93219 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.790, 71.790, 129.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.310 - 2.405 |
| R-factor | 0.1622 |
| Rwork | 0.155 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ow5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BUSTER |
| Refinement software | BUSTER-TNT (2.10) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.310 | 30.000 | 2.491 |
| High resolution limit [Å] | 2.405 | 5.160 | 2.405 |
| Rmerge | 0.119 | 0.074 | 0.377 |
| Total number of observations | 41467 | ||
| Number of reflections | 13267 | ||
| <I/σ(I)> | 6.21 | 2.32 | |
| Completeness [%] | 96.2 | 92.8 | 89.19 |
| Redundancy | 1.8 | 3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 301.15 | In vivo crystals, grown directly in insects |
