4X1Y
Discovery of cytotoxic Dolastatin 10 analogs with N-terminal modifications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.610, 127.860, 255.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.590 - 3.190 |
R-factor | 0.1926 |
Rwork | 0.188 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hkb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.190 | 8.670 | 3.190 |
Rmerge | 0.104 | 0.068 | 0.832 |
Total number of observations | 164538 | ||
Number of reflections | 36225 | ||
<I/σ(I)> | 4.7 | ||
Completeness [%] | 97.5 | 95.1 | 90.4 |
Redundancy | 4.5 | 4.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 5% PEG 400, 0.1M LiSO4, 5-7% PEG 20K, 50 mM K-PIPES |