4X1T
The crystal structure of Arabidopsis thaliana galactolipid synthase MGD1 in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 63 |
| Unit cell lengths | 139.250, 139.250, 38.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.580 - 2.250 |
| R-factor | 0.1994 |
| Rwork | 0.199 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wyi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.042 | 0.532 |
| Number of reflections | 20423 | |
| <I/σ(I)> | 20.8 | 2.9 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-18% ethylene glycol |
