4X1O

Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase from Catenulisporales acidiphilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	42.385, 66.113, 80.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.495 - 1.700
R-factor	0.1808
Rwork	0.177
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mq2
RMSD bond length	0.007
RMSD bond angle	1.088
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.072
Number of reflections	25598
<I/σ(I)>	32	2.2
Completeness [%]	99.0	98.4
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	0.03 M BisTris, 25.5 % PEG3350