4X1M
Structural basis for mutation-induced destabilization of Profilin 1 in ALS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-18 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.650, 31.712, 60.539 |
Unit cell angles | 90.00, 122.03, 90.00 |
Refinement procedure
Resolution | 19.282 - 2.170 |
R-factor | 0.1973 |
Rwork | 0.197 |
R-free | 0.21390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fik |
RMSD bond length | 0.003 |
RMSD bond angle | 0.668 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.036 | 0.095 |
Number of reflections | 6468 | |
<I/σ(I)> | 8.8 | 18.3 |
Completeness [%] | 99.5 | 100 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PFN1 crystals were grown by hanging drop vapor diffusion after mixing the PFN1 protein with a 1:1 ratio of reservoir solution at 298K for E117G. Reservoir solution for E117G contained 50 mM KH2PO4, 41% (wt/vol) PEG 8,000 and 100 mM MES |