4X1H
Opsin/G(alpha) peptide complex stabilized by nonyl-glucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.136, 242.136, 109.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.907 - 2.290 |
| R-factor | 0.2177 |
| Rwork | 0.217 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cap |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.878 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.910 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.026 | 0.850 |
| Number of reflections | 57205 | |
| <I/σ(I)> | 56.7 | 1.2 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 3.1M ammonium sulfate, 100mM citrate |
