4X0V
Structure of a GH5 family lichenase from Caldicellulosiruptor sp. F32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-30 |
Detector | RIGAKU |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.659, 157.780, 120.803 |
Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
Resolution | 46.650 - 2.798 |
R-factor | 0.1843 |
Rwork | 0.183 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1edg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.231 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.798 |
Number of reflections | 78399 |
<I/σ(I)> | 11.5 |
Completeness [%] | 99.8 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Li citrate tribasic, 20% PEG 3350 |